Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Expression of bone morphogenetic protein-2 and its receptors in epithelial ovarian cancer and their influence on the prognosis of ovarian cancer patients

doi: 10.1186/1756-9966-29-85

Figure Lengend Snippet: Correlation of the expression of BMP-2 and its receptors with survival rate and survival time of ovarian cancer patients

Article Snippet: BMP-2 rabbit anti-human polyclonal antibody, SABC immunohistochemical kit, DAB color-developing reagent (Wuhan Boster, China); β-actin rat anti-human polyclonal antibody, BMPRIA rabbit anti-human polyclonal antibody, BMPRIB rabbit anti-human polyclonal antibody, BMPRII goat anti-human polyclonal antibody (Santa Cruz, USA); BMP-2, BMPRIA, BMPRIB, BMPRII and internal reference β-actin primers (Shanghai Sangon, China); Trizol total RNA extraction reagent (Invitrogen, USA); RT kit and PCR amplification reagents (TaKaRa-Dalian, China); alkaline phosphatase-labeled goat anti-rabbit IgG, rabbit anti-goat IgG (Sigma, USA); Bradford protein kit (Nanjing Keygen, China); nitrocellulose filter (Shanghai Gene, China), β-naphthyl acid phosphate, O-dianisidine (tetrazotized; Sigma, USA).

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Dose-dependent inhibitory effects of zoledronic acid on osteoblast viability and function in vitro

doi: 10.3892/mmr.2015.4627

Figure Lengend Snippet: Effect of ZA on the expression of BMP-2 and phosphorylation of the ERK 1/2 and p38 pathways in MC3T3-E1 cells. The MC3T3-E1 cells were incubated with various concentrations of ZA (0–1 µ M) for 7 days. (A) Levels of secreted BMP-2, measured using an ELISA. (B) mRNA expression levels of BMP-2, determined using reverse transcription-quantitative polymerase chain reaction analysis. (C) Protein levels of BMP-2, p38, p-p38, ERK 1/2 and p-ERK 1/2, detected using western blot analysis and normalized to GAPDH. (D) Quantitative analysis of the blots for BMP-2. (E) Relative expression of p-ERK, normalized to ERK, and relative expression of p-p38 normalized to p38. The results are expressed as the mean ± standard error of the mean (n =3 for each group). * P<0.05, ** P<0.01 and *** P<0.001, compared with the 0 µ M group. BMP-2, bone morphogenetic protein 2; ERK 1/2, extracellular signal-regulated kinase 1/2; p-, phosphorylated; ELISA, enzyme-linked immunosorbent assay.

Article Snippet: Primary antibodies against the following targets were used: Monoclonal rabbit anti-p38 antibody (1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA; cat. no. 8690), monoclonal rabbit anti-phosphorylated (p)-p38 antibody (1:1,000; Cell Signaling Technology, Inc.; cat. no. 4511), monoclonal rabbit anti-ERK 1/2 antibody (1:1,000; Cell Signaling Technology, Inc.; cat. no. 4695), monoclonal rabbit anti-p ERK 1/2 antibody (1:1,000; Cell Signaling Technology, Inc.; cat. no. 4370), polyclonal rabbit anti-inactive caspase-3 antibody (1:500; Santa Cruz Biotechnology, Inc., Dallas, TX, USA; cat. no. SC-7148), polyclonal rabbit anti-OCN antibody (1:500; Santa Cruz Biotechnology, Inc.; cat. no. SC-30045), polyclonal rabbit anti-active caspase-3 antibody (1:200; Abcam, Cambridge, UK; cat. no. ab2302), monoclonal rabbit anti-ALP antibody (1:20,000; Abcam; cat. no. ab108337), polyclonal rabbit anti-BMP-2 antibody (1:1,000; Abgent Biotech Co., Ltd., Suzhou, China; cat. no. AP13858c), polyclonal rabbit anti-Runx2 antibody (1:400; Wuhan Boster Biological Technology, Ltd.; cat. no. BA3613-2), monoclonal mouse anti-glyceraldehyde phosphate dehydrogenase (GAPDH) antibody (1:400; Wuhan Boster Biological Technology, Ltd.; cat. no. BM1623), monoclonal mouse anti-β actin antibody (1:400; Wuhan Boster Biological Technology, Ltd.; cat. no. BM0627).

Techniques: Expressing, Incubation, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Western Blot